Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
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Abstract
Tuberculosis (TB) has long been a significant threat to humanity, affecting health, society and the economy. Recently, the Belagavi district experienced a decline in TB notifications amid the COVID‐19 pandemic, potentially leading to increased morbidity, mortality and household transmission. New sputum samples with prior consent and detailed patient histories were collected from the Respiratory OPD of Dr Prabhakar Kore Hospital and MRC. Petroff’s decontamination was performed on the samples in a BSL‐2 cabinet, followed by ZN‐stain and Auramine O’ stain screening under compound and fluorescent microscopes, respectively. PCR targeting IS6110 was conducted on the remaining samples after storage at (‐80 ), following DNA extraction using Qiagen Kit. Standardization included preparation of Master mix (Takara) and PCR procedure with specific primers. Gel Electrophoresis identified amplified products showing a band at 123 bp as MTB. Data analysis was employed. The study identified a majority (38%) of positive MTB cases in the 31‐ 40 years age group using conventional PCR, with lower rates observed in younger age groups. Syndemic effects of coexisting DM and HIV were evident in 5.13% of patients. PCR demonstrates considerable potential for Mycobacterium tuberculosis detection from sputum samples, offering a safe and effective diagnostic alternative to the traditional staining techniques, despite limitations in throughput and cost‐effectiveness in rural populations.
How to cite this article:
Sneha Roy Choudhury Mukherjee. Detection of Mycobacterium tuberculosis by Polymerase Chain Reaction: A one‐year Cross Sectional Study in the Rural Population of Belagavi District.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.11.280.289
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.11.280.289