Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
Abstract
The high prevalence of nosocomial infections is related to the use of medical insertion devices such as central venous catheters (CVCs). Most of the microorganisms causing nosocomial infections are biofilm producers, this characteristic allows them to adhere to abiotic surfaces and cause initial catheter infections. Disinfectants with broad range multiple target activity are widely used in hospitals for skin antisepsis in order to successfully prevent nosocomial infections. The clinicians can combat catheter‐related infections earlier if appropriate disinfectant with right strength and contact duration are used. This study was conducted to evaluate the effect of disinfectants i.e., 0.5% chlorhexidine, 70%alcohol and povidone‐iodine against various biofilm producing organisms among catheter‐related infections. A total of 106 catheter samples obtained from 105 patients were processed by standard microbiological methods. The micro titre plate method was used to detect biofilm formation and the effect of disinfectants was studied by incubating biofilms with 0.5 percent chlorhexidine, 70% alcohol and povidone‐iodine for 1, 5 and 10 minutes. Of the 106 catheters, 53 isolates were obtained. Out of 53 clinical isolates. 96.2% were found to produce biofilm. Chlorhexidine was highly effective against CONS (100%) after 1 and 5 min followed by gram negative bacteria (90.9%) after 1 min of incubation. 70% Alcohol was more effective against CONS (90.9%) after 1 min compared to gram negative bacteria and Candida. Povidone iodine reduced biofilm in 72.7% CONS after 5 min of contact time but around 50% or less gram negative and Candida isolates showed reduction.
How to cite this article:
H. Kavitha, Pooja Mansabdar, B. Amrutha Kumari and H. Kavitha. The Impact of Different Disinfectants on Microbial Biofilm Formation in Catheter Related Infections.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.11.163.169
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.11.163.169