Research Journal of Applied Sciences
ISSN: Online 1993-6079ISSN: Print 1815-932x
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Morphology of Fresh and Cryopreserved Sperms of Banana Shrimp Penaeus merguiensis (De Man, 1888)
A.J. Memon, A.D. Talpur, M.I. Khan, M.O. Fariddudin, J. Safiah , A.B. Abol-Munafi and M. Ikhwanuddin
Page: 54-59 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
The spermatophore morphology of the P. merguiensis from Kedah, Malaysia is described. About 10 cryopreserved groups as 6, 12 and 24 h, 7, 30, 60, 90, 120, 150 and 180 days and fresh as control was examined from each of three replication were evaluated for sperm gross morphology evaluations. A fully mature male’s broodstock of P. merguiensis was taken with fresh spermatophores and evaluated for sperm morphologically. Cryopreserved spermatophore after the thawing 27°C/2 min (fresh and frozen) individually transferred into glass homogenizer (High speed variable speed reversible, Glas-col, Terre Havte In USA) with 200 μL of Ca-F saline. Fixation, dehydration by series of alcohol, Critical Point Dry (CPD) and mount specimens on to stubs using or carbon dots as well as using Auto Fine Coater and Sputter Coater moreover scanning by Model JEOL 6360LA scanning electron microscope. The cryopreserved spermatophore shows similarities with those of fresh, there were no significant differences (p>0.05) between the freshly spermatophore and spermatophorestored up to 90 days at -196°C liquid nitrogen.
How to cite this article:
A.J. Memon, A.D. Talpur, M.I. Khan, M.O. Fariddudin, J. Safiah , A.B. Abol-Munafi and M. Ikhwanuddin. Morphology of Fresh and Cryopreserved Sperms of Banana Shrimp Penaeus merguiensis (De Man, 1888).
DOI: https://doi.org/10.36478/rjasci.2012.54.59
URL: https://www.makhillpublications.co/view-article/1815-932x/rjasci.2012.54.59