Research Journal of Biological Sciences
ISSN: Online 1993-6087ISSN: Print 1815-8846
Abstract
Destruxins, a family of cyclic peptide mycotoxins, have been being paid more and more attention for their multiple bioactivities such as insecticide, antivirus and immunomodulation. In order to decrease the consumption of solvents and time to isolate and purify destruxins A and B (DA and DB), Ultrasonic Wave Extraction (UWV) and Vacuum Liquid Chromatography (VLC) were employed. Under the optimal conditions of pH 4-5, solvent (dichloromethane/ethyl acetate, v v 1 = 1/1)/broth (v v 1) between 1-1.5 and extracted time in 45-90 min, UWV gave an excellent recovery (extracting efficiency > 90%). Meanwhile, in the VCL experiment, the gradient of hexane/acetone 100 /0-93/7 as eluant could isolate DA and DB clearly. However, the eluants of dichloromethane/methanol and hexane/ethyl acetate were not as good as hexane/acetone.
How to cite this article:
Qiongbo Hu , Shunxiang Ren , Di Xu and Shuyan Liu . The Optimization of Ultrasonic Wave Extraction and Vacuum Liquid Chromatography for Isolation of Destruxins.
DOI: https://doi.org/10.36478/rjbsci.2007.462.467
URL: https://www.makhillpublications.co/view-article/1815-8846/rjbsci.2007.462.467