Journal of Food Technology
ISSN: Online 1993-6036ISSN: Print 1684-8462
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Application of Statistical Method for Screening of Factors Influencing the Production of #-cyclodextrin from Sago Starch Using Combination of Pullulanase and CGTase Enzymes
Suzana Muhamad Taib , Wan Zawiah Wan Zin , Marina Zahari Rosli Md.Illias and Osman Hassan
Page: 538-541 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
The production of $-cyclodextrin ($-CD) from sago starch was carried out using combination of debranching enzyme and Cyclodextrin Glucanotransferase (CGTase). The contribution of each reaction parameters was evaluated and optimised using statistical modelling. A Fractional Factorial Design (FFD) was employed to screen the effect of substrate concentration, CGTase concentration, pullulanase concentration, pH and temperature for CGTase reaction on the production of $-cyclodextrin ($-CD) using combination of pullulanase and CGTase. The result of first-order factorial design showed that pH and pullulanase had significant positive effect (p<0.05) to the reaction. Substrate concentration, CGTase concentration and temperature exhibited insignificant effect in this reaction. In addition, interaction between CGTase and pH, substrate and pH, pullulanase and temperature, CGTase and temperature, substrate and CGTase gave significant effects (p<0.05) to the $-CD production.
How to cite this article:
Suzana Muhamad Taib , Wan Zawiah Wan Zin , Marina Zahari Rosli Md.Illias and Osman Hassan . Application of Statistical Method for Screening of Factors Influencing the Production of #-cyclodextrin from Sago Starch Using Combination of Pullulanase and CGTase Enzymes.
DOI: https://doi.org/10.36478/jftech.2005.538.541
URL: https://www.makhillpublications.co/view-article/1684-8462/jftech.2005.538.541