Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Molecular Cloning, Characterization and Tissue Distribution of MyoGenin Genein Goose (Anser cygnoides)
Daqian He, Shaoming Gong, Weihu Chen, Sifeng Yi, Chuang Li, Huiying Wang, Yunzhou Yang, Yi Liu and Cui Wang
Page: 393-398 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
MyoGenin (MyoG) is a basic Helix-Loop-Helix (bHLH) transcription factor that belongs to the Muscle-specific transcription Factors (MRFs) family which plays critical roles in regulating the skeletal muscle development and growth. In this study, the complete coding sequence and genomic DNA sequence of goose MyoG gene were cloned and characterized. The goose MyoG CDS was composed of 684bp that encoded a 227 amino acid protein, including a highly conserved basic helix-loop-helix domain. Multiple sequence alignments and phylogenetic analysis indicated that the deduced goose MyoG protein was conserved in vertebrates, especially in the avian species. The goose MyoG genomic DNA sequence we obtained was 3444bp and consisted of 3 exons and 2 introns.Sem-quantitative RT-PCR analysis demonstrated that the goose MyoG mRNA was specifically expressed in the breast muscle and leg muscle tissues, little or no expression was observed in heart, liver, spleen, lung, kidney, muscular stomach, brain, intestine and sebum. These data will serve as a foundation for further insight into the functions of the MyoG gene in Chinese domestic goose.
How to cite this article:
Daqian He, Shaoming Gong, Weihu Chen, Sifeng Yi, Chuang Li, Huiying Wang, Yunzhou Yang, Yi Liu and Cui Wang. Molecular Cloning, Characterization and Tissue Distribution of
MyoGenin Genein Goose (Anser cygnoides).
DOI: https://doi.org/10.36478/javaa.2015.393.398
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2015.393.398