Abstract

In this study, we screened the possible differentially expressed genes in bovine Embryonic Stem Cell (ESC)-like cells with different clone morphologies and passages via Differential Display Reverse Transcription PCR (DDRT-PCR) technique in combination with sequence analysis and GenBank BLAST. The results showed that among the bovine ESC-like cells with different clone morphologies, six differentially expressed fragments were identified highly homological with six known genes, namely: RPL9, LOC100850994, AMP, RPL31, Erbb2ip and CLIP1. Real-time quantitative PCR (qRT-PCR) results revealed that the expression levels of RPL9, LOC100850994 and RPL31 genes were significantly different among the bovine ESC-like cells with slate, block and bubble clone morphologies and Western blotting data showed that the expression levels of RPL9 were significantly different among the cells with the three clone morphologies. From the bovine ESC-like cells of different passages, seven differentially expressed fragments were identified highly homological with seven other known genes, namely: IK, TKDP1, BZW, PRL9, RPL31, RP42 and IGBP1. qRT-PCR results revealed that the expression levels of IK, TKDP1, BZW, PRL9, RP42 and IGBP1 were significantly different among the bovine ESC-like cells of 1st and 10th passages. Western blotting data showed that protein expression levels of all these genes were significantly higher in passage 1 bovine ESC-like cells than passage 10 cells.