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Journal of Animal and Veterinary Advances

ISSN: Online 1993-601X
ISSN: Print 1680-5593
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Effect of the Crude Polysaccharides from Poria cocos on mRNA Expression of Adipogenic Factors During 3T3-L1 Preadipocyte Differentiation

Xun Wang, Hengmin Cui, Haifeng Liu, Ling Zhao, Xi Peng, Hengyong Xu, Yan Wang, Yuhao Wang, Ling Niang and Qian Rong
Page: 1312-1316 | Received 21 Sep 2022, Published online: 21 Sep 2022

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https://doi.org/10.36478/javaa.2013.1312.1316

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Abstract

To study the effect of the crude polysaccharides from Poria cocos (PCCP) on mRNA expression of adipogenic factors during 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were induced to differentiate with MDI (3-isobutyl-1-Methylxanthine, Dexamethasone and Insulin) induction media contained PCCP (50 μg mL-1) and cultured for 8 days. The extent of the differentiation was assessed by Nile Red staining and GPDH activity. In addition, researchers used real-time quantitative PCR to analyzed the expression of genes related to adipocyte differentiation. The results showed that treatment of differentiating 3T3-L1 preadipocytes with PCCP (50 μg mL-1) resulted in an increase in the expression of the adipocyte genes such as Fatty Acid System Synthase (FAS), adipocyte fatty acid binding Protein (aP2), insulin-responsive Glucose Transporter (GLUT4) and transcriptional factor genes such as CCAAT/Enhancer Binding Protein α (C/EBPα), peoxisome Proliferator-Activated Receptor gamma (PPARγ) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) to some degree. Overall, the data suggested that there was the tendency of increasing the expression of genes related to adipocyte differentiation in PCCP treated cells.

How to cite this article:

Xun Wang, Hengmin Cui, Haifeng Liu, Ling Zhao, Xi Peng, Hengyong Xu, Yan Wang, Yuhao Wang, Ling Niang and Qian Rong. Effect of the Crude Polysaccharides from Poria cocos on mRNA Expression of Adipogenic Factors During 3T3-L1 Preadipocyte Differentiation.
DOI: https://doi.org/10.36478/javaa.2013.1312.1316
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2013.1312.1316