Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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The Ubiquitin C-Terminal Hydrolase L-1 (PGP9.5) as a Suitable Marker to Trace and Isolate Spermatogonia Cells from Ovine Testis
Muren Herrid, Bou Shorgan, Xin Wang, Zhiying He, Uyunbilig Borjigin and Sachula Wu
Page: 4364-4368 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
The Ubiquitin C-terminal Hydrolase L-1 (UCH-L1) is usually named as PGP9.5. Earlier study indicated that PGP9.5 has been used to identify type A spermatogonia in some species such as mouse and pig. In the present study, the localization of PGP9.5 was studied on paraffin sections of the ram testis samples in order to understand the correlation between gonocyte migration and spermatogonia location in the seminiferous tubules of ovine testis development. The results also indicated that testes from neonatal ovine had only one type of undifferentiated germ cell gonocyte and PGP9.5 as a spermatogonia cell marker was suitable for isolation and enrichment of ovine Spermatogonial Stem Cells (SSCs). Thus, the finding will be helpful for the future study on ovine SSCs in long-term culture and transplantation.
How to cite this article:
Muren Herrid, Bou Shorgan, Xin Wang, Zhiying He, Uyunbilig Borjigin and Sachula Wu. The Ubiquitin C-Terminal Hydrolase L-1 (PGP9.5) as a Suitable Marker to Trace and Isolate Spermatogonia Cells from Ovine Testis.
DOI: https://doi.org/10.36478/javaa.2012.4364.4368
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.4364.4368