Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Expression of the B Subunit of Escherichia coli Heat-Labile Enterotoxin in Transformed Bombyx mori BmN Cells
Hong-Biao Weng, Cheng-Liang Gong, Ai-Hong Ye, Jin-Ru Cao, Wen-Lin Zhou, Li-Hua He, Ren-Yu Xue, Guang-Li Cao and Yong-Qiang Wang
Page: 3785-3791 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
The non-toxic B subunit of Escherichia coli heat-labile enterotoxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered antigens. To obtain transformed silkworm cell line stably expressing LTB, researchers fused the LTB coding sequence, neomycin-resistance gene (NeoR) and gfp gene into piggyBac-based transponson vector and transduced into silkworm BmN cells. After screening against antibiotic G418, the positive rate of cells emitting green fluorescence was 70.79%. PCR detection indicates the existence of exogenous LTB coding sequence, NeoR and gfp in the transformed cell genome. Western blot analysis also confirms the predicated ~60 kDa band of LTB protein. These results demonstrated that the strategy was practicable.
How to cite this article:
Hong-Biao Weng, Cheng-Liang Gong, Ai-Hong Ye, Jin-Ru Cao, Wen-Lin Zhou, Li-Hua He, Ren-Yu Xue, Guang-Li Cao and Yong-Qiang Wang. Expression of the B Subunit of Escherichia coli Heat-Labile Enterotoxin in Transformed Bombyx mori BmN Cells.
DOI: https://doi.org/10.36478/javaa.2012.3785.3791
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.3785.3791