Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
83
Views
0
Downloads
Immunogenicity of Foot and Mouth Disease Virus Type Asia 1 Protein VP1-2A Fused with a Multi-Epitope Expressed in Pichia pastoris
Zhan-Bo Zhu, Ying Xi , Bo Hu , Chang Li , Huijun Lu , Kuoshi Jin , Jingqiang Ren , Cunxia Liu , Minglan Jin and Ningyi Jin
Page: 1512-1517 | Received 21 Sep 2022, Published online: 21 Sep 2022
Full Text Reference XML File PDF File
Abstract
Foot and Mouth Disease (FMD) is a highly infectious and economically devastating disease of livestock. A genetically engineered vaccine against Foot and Mouth Disease Virus (FMDV) may be a good control measure for use during an epidemic. In this study, a secretory expression vector, pPIC9K-VP1-2A-CTE, containing a fusion of the bovine (FMDV) type Asia 1 VP1-2A gene and a multi-epitope CTB-TEpi (CTE) was constructed. After being linearized with Sal I, the recombinant plasmid was transformed into Pichia pastoris GS115 by electroporation. The fusion protein expressed in yeast was analyzed by SDS-PAGE and Western blot and immunogenicity of the fusion protein was assessed. The expressed recombinant VP1-2A and CTE protein elicited specific humoral and cellular immune responses in mice. Additionally, immunized guinea pigs were protected when challenged with the FMD Asia 1 virus.
How to cite this article:
Zhan-Bo Zhu, Ying Xi , Bo Hu , Chang Li , Huijun Lu , Kuoshi Jin , Jingqiang Ren , Cunxia Liu , Minglan Jin and Ningyi Jin . Immunogenicity of Foot and Mouth Disease Virus Type Asia 1 Protein VP1-2A Fused with a Multi-Epitope Expressed in Pichia pastoris.
DOI: https://doi.org/10.36478/javaa.2012.1512.1517
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.1512.1517