Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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A Semi-Quantitative RT-PCR to Assess Differential Expression Levels of TCF3 Gene in Two Chinese Indigenous Chicken Breeds
Wang Jin-yu , Bian Liang-yong , Yang Yan , Li lin-Chuan , H. Hassan Musa and M. Dafalla Mekki
Page: 185-189 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
Semi-Quantitative Polymerase Chain Reaction (RT-PCR) was applied to quantify and compare variations in avian TCF3 expression level between two Chinese indigenous chicken breeds for different tissues. Six primer combinations in addition to -actin as an internal control were used. The expression level of TCF3 was greater in Jinghai breed than Suqin breed for different tissues. The variability of gene expression between two breeds showed 18.55, 37.85 and 66.15% higher expression in Jinghai breed than Suqin breed for kidney, lung and spleen tissues, respectively. Differences were found to be significant (p<0.05) only for expression level in lung and spleen tissues. Significant effect of sex upon TCF3 expression was detected for both breeds.
How to cite this article:
Wang Jin-yu , Bian Liang-yong , Yang Yan , Li lin-Chuan , H. Hassan Musa and M. Dafalla Mekki . A Semi-Quantitative RT-PCR to Assess Differential Expression Levels of TCF3 Gene in Two Chinese Indigenous Chicken Breeds.
DOI: https://doi.org/10.36478/javaa.2007.185.189
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2007.185.189