Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
Abstract
Tyrosine Hydroxylase (TH) and Choline Acetyltransferase (ChAT) immunoreactivity were examined in the adult mouse brain by a modified double labelling immunofluorescence method. Mice were transcardially perfused with saline followed by fixative (4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4). Frozen brain was cut at 30 m thickness for light microscopy. All staining procedures were conducted under room temperature. Dopaminergic neurons were identified from Cy2-coupled-donkey-anti-rabbit antibody and cholinergic neurons by Cy3-coupleddonkey-anti-goat for ChAT. The cell bodies of both TH- and ChAT- positive neurons with their respective axonal and dendritic processes were easily visible. This method proved reliable for simultaneous demonstration, in the same brain tissue sections, of TH- and ChAT-containing neurons.
How to cite this article:
A.O. Ihunwo and R. Schliebs . Simultaneous Identification of Tyrosine Hydroxylase and Choline Acetyltransferase Neurons.
DOI: https://doi.org/10.36478/javaa.2007.1116.1119
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2007.1116.1119