Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Analysis of of Escherichia coli O157 isolated from imported beef and lamb in Malaysia and United Arab Emirates using RAPD-PCR
Sara Abudafeera , Mariana Nor Shamsudin , Son Radu , Rozita Rusli and Osman Elobied
Page: 148-153 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
Enterohemorrhagic Escherichia coli (EHEC) of serotype O157 causing hemorrhagic colitis is on the increase in many parts of the world, hence specific surveillance of this pathogen is essential for identifying the sources and monitoring its spread. In this study, a total of 22 imported beef isolates of E. coli O157 isolated from wet market in Seri Serdang in Malaysia and 24 imported lamb isolates of E. coli O157 isolated from Center Slaughterhouse in Al-Ain in United Arab Emirates were examined. All the isolates were investigated by randomly amplified polymorphic DNA (RAPD) analysis with one primer: Gen1-50-9 (5?-AGAAGCGATG-3?). The primer generated polymorphisms in most of the isolates of E. coli O157 tested, producing bands ranging from 0.25 to 4.0 kilobases. The RAPD profiles revealed a high level of DNA sequence diversity within E. coli O157 isolates tested.
How to cite this article:
Sara Abudafeera , Mariana Nor Shamsudin , Son Radu , Rozita Rusli and Osman Elobied . Analysis of of Escherichia coli O157 isolated from imported beef and lamb in Malaysia and United Arab Emirates using RAPD-PCR.
DOI: https://doi.org/10.36478/javaa.2004.148.153
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2004.148.153