In recent decades, there has been a growing interest about the role of gram negative bacteria in sputum samples. Identifying gram negative bacteria and in particular resistant gram negative bacteria is of paramount importance in patients with higher clinical severity and unfavorable outcomes. The emerging pathogens were isolated by culture and antibiotic susceptibility testing. To classify the various Gram-negative bacteria, biochemical tests namely indole, citrate utilization, triple sugar iron (TSI), urease and oxidase were performed. Sputum samples of patients were collected and cultured for detection of microorganisms and further subjected to Antibiotic sensitivity and biochemical tests in the Department of Microbiology, KPC Medical College and Hospital, Jadavpur, Kolkata. During the study period 1 year, a total of 200 sputum samples were received for bacterial culture. In our study, 143 were gram negative bacteria and rest of the samples were either gram positive or environmental isolates. Out of these, 65 samples were found to be multidrug resistant Gram negative bacteria. In this study, four Gram-negative bacteria namely Klebsiella pneumoniae, Pseudomonas sp., E. coli and Proteus sp. were isolated from sputum samples of patients.
Tamasi Mukherjee, Suman Nandi and Mayur Bahan Mukherjee. Emerging Drug Resistance in Sputum-Derived Gram Negative Pathogens.
DOI: https://doi.org/10.36478/10.59218/makrjms.2024.2.18.551.554
URL: https://www.makhillpublications.co/view-article/1815-9346/10.59218/makrjms.2024.2.18.551.554