Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
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Comparative Patterns of Antimicrobial Susceptibility Testing by Direct Susceptibility and Conventional Suscepbility Testing of Blood Culture Isolates from a Tertiary Care Centre from Dibrugarh, Assam
Chimanjita Phukan, Prapti Bora, Sumita Boro, Arunjyoti Sarmah, Rupsikha Gogoi and Reema Nath
Page: 168-173 | Received 05 Nov 2024, Published online: 21 Jan 2025
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Abstract
Early initiation of appropriate antimicrobial agents is essential for adequate management of septicemia. Antimicrobial susceptibility reporting by conventional methods require a minimum of 24‐48 hours after flagging positive. Direct antimicrobial susceptibility testing can reduce the turn around time by 24 hours. To compare the difference in susceptibility patterns of blood culture isolates between conventional antimicrobial susceptibility testing and primary drug sensitivity method in sepsis cases and to find the very major, major and minor errors in the antibiotics tested for Gram negative and Gram positive isolates, amongst the two methods. Blood culture bottles flagged positive by BacT/ALERT systems were analyzed by Grams stain. Samples showing monomicrobial growth were further processed for primary drug susceptibility testing by disk diffusion method, following the CLSI 2022 guidelines. Conventional culture and sensitivity testing were also carried out simultaneously. The number of very major errors, major errors and minor errors were calculated for each antimicrobial agent, along with the concordance rate between conventional and direct susceptibility testing. Out of 120 positive cultures, 68 (56.7%) were Gram negative isolates and 44 (36.7%) were Gram positive isolates. Most commonly isolated Gram negative and Gram positive organisms were Escherichia coli and Staphylococcus aureus, respectively. Slightly high discrepencies were seen for Enterobacterales testing against Ciprofloxacin, Meropenem and Beta lactam/Beta lactamase inhibitors, non enterobacterales against Ampicillin/sulbactam, Meropenem and Amikacin, Staphylococci species against Cefoxitin and Enterococci species against Ampicillin and Tetracycline. Primary drug susceptibility testing enables physicians to initiate appropriate antimicrobial therapy at least 24 hours earlier compared to conventional methods. This can significantly reduce mortality in patients with septicemia.
How to cite this article:
Chimanjita Phukan, Prapti Bora, Sumita Boro, Arunjyoti Sarmah, Rupsikha Gogoi and Reema Nath. Comparative Patterns of Antimicrobial Susceptibility Testing by Direct Susceptibility and Conventional Suscepbility Testing of Blood Culture Isolates from a Tertiary Care Centre from Dibrugarh, Assam.
DOI: https://doi.org/10.36478/10.36478/makrjms.2025.2.168.173
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2025.2.168.173