Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
Abstract
The Aim of the study is to Describe the Dysbiosis of Skin Microbiota And Diversity of Increased Fungal Infection Associated with Severity of Disease In Atopic Dermatitis. Using shotgun metagenomics we characterized microbial compositions from 14 well defined skin sites from 10 patients with Atopic Dermatitis and 5 healthy controls. We found clear differences in microbial composition between AD and controls at multiple skin sites, most pronounced on the flexures and neck. The flexures exhibited lower alpha‐diversity and were colonized by S. aureus, accompanied by S. epidermidis in lesions. Malassezia species were absent on the neck in AD. In lesional samples, both the genus Staphylococcus and Staphylococcus phages were more abundant. S. aureus abundance was higher across all skin sites except from the feet. In samples where S. aureus was highly abundant, lower abundances of S. hominis and Cutibacterium acnes were observed. M. osloensis and M. luteus were more abundant in AD. By single nucleotide variant analysis of S. aureus we found strains to be subject specific. On skin sites some S. aureus strains were similar and some dissimilar to the ones in the nares. Similar to previous studies, we found an increased fungal diversity in AD patients. Interestingly, it was only increased in patients with severe AD and not in mild‐to‐moderate cases. We were unable, however, to observe the previously described decrease of bacterial diversity in severe AD. The bacterial diversity was comparable in all three study groups and was even slightly elevated in mild‐to‐moderate AD. An explanation for this discrepancy might be that we analysed a‐diversities at the class and genus rather than at the species or the amplicon sequence variants (ASVs) level because the V1‐3 region of the 16S rRNA gene operon that we used for analyses does not allow proper resolution at the species level or beyond. In Our Study a global and site‐specific dysbiosis in AD, involving both bacteria, fungus. When defining targeted treatment should both consider the individual and skin site. we found an increased fungal diversity in AD patients. Interestingly, it was only increased in patients with severe AD and not in mild‐moderate cases. We were unable, however, to observe the previously described decrease of bacterial diversity in severe AD.
How to cite this article:
Dhwani Alok Saxena, Rahul Balmiki and Pawan Singh. Dysbiosis of Skin Microbiota with Increased Fungal Diversity is Associated with Severity of Disease in Atopic Dermatitis.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.10.386.393
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.10.386.393