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Journal of Animal and Veterinary Advances

ISSN: Online 1993-601X
ISSN: Print 1680-5593
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Influence of Hormones on mRNA Abundance of Enzymes Related Lipid Metabolism in Longissimus Dorsi Muscle in vitro

Gao Teng-Yun, Gao Min, Lu De-Xun and Lian Hong-Xia
Page: 386-392 | Received 21 Sep 2022, Published online: 21 Sep 2022

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https://doi.org/10.36478/javaa.2012.386.392

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Abstract

The current study was performed to examine the response of the enzymes related lipid metabolism gene to hormonal stimulation in pig muscle tissue from finishing pigs. Longissimus dorsi muscle was collected and muscle tissue explants (approximately 100 mg) were prepared by sterile technique in finishing barrows (100.55.3 kg BW) of Rongchang. A in vitro system was established to investigate the regulation of Insulin (INS), Somatotropin (ST), Epinephrine (Epi) and Glucagon (GCG) on Lipoprotein Lipase (LPL), Acetyl-CoA Carboxylase (ACC), Fatty Acid Synthase (FAS) and Hormone Sensitive Lipase (HSL) gene expression to determine whether there was any potential for the manipulation of the Intramuscular Fat (IMF) deposit in Longissimus dorsi muscle of pig. The experiment was conducted with an orthogonal design with a L9(34) and each treatment had three replicates. Results showed that ST significantly affected HSL and FAS mRNA abundance (p<0.01) and INS influenced LPL and ACC mRNA abundance (p<0.01), respectively. This results suggested that the combination of hormones (INS; 1x10–5 M, ST; 5x10–11 M, Epi; 1x10–6 M, GCG; 1x10–7 M, INS; 1x10–7 M, ST; 5x10–9 M, Epi; 1x10–6 M, GCG; 1x10–6 M, INS; 1x10–7 M, ST; 5x10–9 M, Epi; 1x10–6 M, GCG; 1x10–6 M) could increase IMF content by increasing LPL, ACC and FAS mRNA expression and at the same time, the combination of hormones (INS; 1x10-7 M, ST; 5x10–10 M, Epi; 1x10–7 M, GCG; 1x10–8 M) had higher IMF content by decreasing HSL mRNA expression. It could be concluded that the combination of hormones (INS; 1x10–5, ST; 5x10–9, Epi; 1x10–7, GCG; 1x10–8) could improve IMF content in finishing pigs.

How to cite this article:

Gao Teng-Yun, Gao Min, Lu De-Xun and Lian Hong-Xia. Influence of Hormones on mRNA Abundance of Enzymes Related Lipid Metabolism in Longissimus Dorsi Muscle in vitro.
DOI: https://doi.org/10.36478/javaa.2012.386.392
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.386.392