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Journal of Animal and Veterinary Advances

ISSN: Online 1993-601X
ISSN: Print 1680-5593
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Hyaluronidase Determination Among Pasteurella multocida Serotype A, B, D and Untypable Isolates

Salmah Ismail
Page: 667-669 | Received 21 Sep 2022, Published online: 21 Sep 2022

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Abstract

A total of 115 Pasteurella multocida isolates were collected from various sources of animals in Malaysia and were purified by Veterinary Diagnostic Laboratory, Petaling Jaya. Isolates were subcultured once on BHI blood agar, incubated at 37?C overnight before amplified in BHI broth. Before carrying out to any further studies, all the isolates were first reconfirmed as Pasteurella multocida by gram staining, colonial morphology and a series of biochemical tests. The cultures of Pasteurella multocida were tested for the production of hyaluronidase by using modification method of Smith and Willett (1968). Hyaluronidase production was demonstrated in all isolates of Pasteurella multocida serotype B, whereas, none was detected in serotype A, D and untypable.


How to cite this article:

Salmah Ismail . Hyaluronidase Determination Among Pasteurella multocida Serotype A, B, D and Untypable Isolates.
DOI: https://doi.org/10.36478/javaa.2004.667.669
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2004.667.669