Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Sequence Analysis of VPg Protein of Asia1 Field Isolates of Foot-and-Mouth Disease Virus in India
S. Biswas , A. Sanyal , D. Hemadri , C. Tosh , S. K. Gupta , M. Mittal , J. K. Mohapatra , G. P. Sabarinath , R. Manoj Kumar and S. K. Bandyopadhyay
Page: 522-526 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
Foot-and-Mouth Disease (FMD), the most economically important disease of cloven-hoofed animals is endemic in India. A total of twenty field isolates belonging to serotype Asia1, collected from different states in India during 1986-2002 were taken as BHK-21 cell culture adapted virus from the National Repository of FMDV at the Project Directorate on Foot-and-Mouth Disease (PD FMD). RT-PCR products of the revived viruses were confirmed by agarose gel electrophoresis and the gel-purified products were subjected to cycle sequencing in an automated sequencer. Vpg protein, which on uridylation serves as a primer during FMDV replication is found conserved across serotypes O, A and C. The sequences generated in the present work were compared with respect to other serotypes with the objective of investigating the amino acid variability of the three redundant copies of VPg proteins in a diverse group field isolates of type Asia1.
How to cite this article:
S. Biswas , A. Sanyal , D. Hemadri , C. Tosh , S. K. Gupta , M. Mittal , J. K. Mohapatra , G. P. Sabarinath , R. Manoj Kumar and S. K. Bandyopadhyay . Sequence Analysis of VPg Protein of Asia1 Field Isolates of Foot-and-Mouth Disease Virus in India.
DOI: https://doi.org/10.36478/javaa.2004.522.526
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2004.522.526