Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
Abstract
Aronia melanocarpa fruit is one of the food sources containing the most anthocyanins, flavonoids, proanthocyanidins, flavanols, phenolic acids and flavonol polyphenols. Studies have shown that this fruit has antitumoral, antiviral, antibacterial, antidiabetic, anti‐inflammatory, antioxidant and antimutagenic properties. In this study; the effects of Aronia melanocarpa extract (AME) on ovarian ischemia reperfusion injury in rats were investigated. For this purpose, Wistar albino female rats (n = 40) were used. Animals were divided into groups as control, ischemia‐reperfusion (IR), Ary1 (low‐dose AME), Ary2 (medium‐dose AME) and Ary3 (high‐dose AME). No substance was administered to the control group. Ovaries of rats in the IR group were subjected to 60 minutes of ischemia and 24 hours of reperfusion. Ary1, Ary2 and Ary3 groups were given AME daily via gavage one week before ischemia (2.5, 5 and 10 mL kg-1, respectively). After the last dose of the extract was applied, bilateral ovarian ischemia (60 min) was created surgically in all treatment groups. Then, these groups were subjected to 24 hours of reperfusion. At the end of the experiment, blood and ovarian tissue were taken from the rats under anesthesia. Malondialdehyde (MDA), catalase (CAT) and super oxide dismutase (SOD) analyzes were performed on the tissue and serum samples taken. All doses of aronia extract did not have a positive effect on MDA and CAT levels (p>0.05). Moreover, the MDA level was higher in the Ary1 group than in the IR group (p<0.05). But the activity of the SOD enzyme increased in all treatment groups. Moreover, the difference was significant in Ary1 and Ary3 groups compared to the IR group (p<0.05). When ovarian tissue was evaluated histopathologically, tissue damage was observed to decrease in the treatment groups. Moreover, as the extract dose increased, it was determined that the ovary gained an appearance close to the control group ovaries. Immunohistochemically, Interleukin‐1 beta, Interleukin‐6 and Tumor necrosis factor alpha reactivity were severe in the IR group. A moderate immunoreactivity was detected in the Ary1 group and a mild immunoreactivity was detected in Ary2 and Ary3. As a result, it can be concluded that high‐dose AME is a substance that can be used for protective purposes against ovarian ischemia‐reperfusion damage.
How to cite this article:
Yadullah Özel, Zahid Paksoy and Nihat Yumusak. Protective Effect of Aronia Melanocarpa Extract on Ischemia Reperfusion Damage in Rat Ovary.
DOI: https://doi.org/10.36478/10.59218/makjava.2023.2.1.12
URL: https://www.makhillpublications.co/view-article/1680-5593/10.59218/makjava.2023.2.1.12