Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Abstract
In this study, we investigated the healing effects of Epigallocatechin gallate (EGCG) on ischemia reperfusion injury in rat ovaries. We used 32 female rats in the study. We randomly divided the animals into 4 groups. The first group was the control group and was anesthetized only. In the second group (IR), experimental ischemia (60 min) and reperfusion (2 hrs) were performed. Animals in the third group (IR+EGCG) were first exposed to ischemia for 60 min. Then, EGCG at a dose of 100 mg kgG1 was administered into the peritoneal cavity. Afterwards, the ovarian tissue was reperfused for 2 hrs. Rats in the fourth group (EGCG) were given EGCG at a dose of 100 mg kgG1 intraperitoneally. Blood and tissue samples were collected under anesthesia at the end of the reperfusion period. We measured TAS and TOS in serum to determine antioxidant capacity and oxidative stress. Histopathological analyzes were performed to examine the ovarian tissue. Additionally, TNF‐immunoreactivity was investigated in the sections taken to determine inflammation. In addition, we demonstrated tissue caspase‐3 levels immunohistochemically to assess apoptosis. No difference was observed between control group and IR group in terms of TAS level (p>0.05). Compared to the control and IR group, TAS level was significantly higher in EGCG treated groups (p<0.05). When TOS levels were compared between groups, the difference was significant and the highest value was in the 3rd group. The oxidative stress index were significantly higher in the IR group compared to the control (p<0.05). EGCG reduced pathological changes such as edema, congestion, vasoconstriction, hemorrhage and necrosis caused by IR. Additionally, the treatment reduced the occurrence of IR‐related inflammation. Polymorphous nuclear cell infiltration was less in rats given EGCG. TNF‐α staining was severe in group 2 and moderate in group 3. Caspase 3 immunoreactivity was very severe in the IR group. In the treatment group, the ovaries were moderate caspase 3 positive. Our findings showed that EGCG has antioxidant, antiapoptotic and antiinflammatory effects and reduces ovarian IR damage. As a result, it was concluded that EGCG treatment can be used in IR injury.
How to cite this article:
Ayşe Eda Ergören, Zahid Paksoy, Nihat Yumusak and Atila Yoldaş. Investigation of the Therapeutic Effects of Epigallocatechin Gallate in the Ovarian Ischemia Reperfusion Model in Rats.
DOI: https://doi.org/10.36478/10.59218/makjava.2023.19.27
URL: https://www.makhillpublications.co/view-article/1680-5593/10.59218/makjava.2023.19.27