TY  - JOUR
T1  - A Consensus Sequence among Natural Transmembrane Linkers for Single-Polypeptide Chain
(SC) Connection of Two G-Protein-Coupled Receptors in Tandem
AU - Kamiya, Toshio AU - Masuko, Takashi AU - Borroto-Escuela, Dasiel Oscar AU - Okado, Haruo AU - Nakata, Hiroyasu 
JO  - Journal of Molecular Genetics
VL  - 13
IS  - 1
SP  - 1
EP  - 45
PY  - 2021
DA  - 2001/08/19
SN  - 2070-4267
DO  - jmolgene.2021.1.45
UR  - https://makhillpublications.co/view-article.php?doi=jmolgene.2021.1.45
KW  - Transmembrane single polypeptide chain linker
KW  -oligomerization
KW  -adenosine A2A receptor
KW  -dopamine D2 receptor; receptor allostery
KW  -fusion protein
KW  -striatum
KW  -supramolecular protein assembly
KW  -consensus sequence of
natural scGPCR TM linker
AB  - A transmembrane (TM) single-polypeptide
chain (sc) linker that can connect any two class A Gprotein-
coupled receptors (GPCRs) in tandem as a genefusion
strategy has been demonstrated. Here, we report
that a natural TM linker for sc exists that can connect two
GPCRs in tandem. <i>In silico</i> analysis indicated that the
central intervening portion (amino acid residues
311~416) of <i>Exaiptasia diaphana</i> (LOC110241027)
GPCR fusion protein contains a single probable TM
domain and that a consensus sequence, T(I/A/P) (A/S)
(L/N) (I/W/L) (I/A/V) GL (L/G) (A/T) (S/L/G) (I/L) is
conserved between this TM and previously characterized
TM-linkers deriving from the type II TM proteins with a
cytoplasmic N-ter segment, single TM and extracellular
C-ter tail, <i>Caenorhabditis elegans odr4</i> and human CD23.
In addition, to the invertebrate TM-linker, it was
found that in the central intervening region of
tropical bird <i>Opisthocomus hoazin</i> protein
LOC104327099 (XP_009930279.1), TM-linker contains
the consensus sequence. Here, we directed for the first
time, our attention to the natural TM sc linker as a rare
evolutionary event among all mammalian and avian
proteins.
ER  -