TY  - JOUR
T1  - Recombinant DNA Technology and its Applications
AU - Choramo, Alemayehu AU - Debelo, Motuma 
JO  - Journal of Molecular Genetics
VL  - 11
IS  - 1
SP  - 1
EP  - 13
PY  - 2019
DA  - 2001/08/19
SN  - 2070-4267
DO  - jmolgene.2019.1.13
UR  - https://makhillpublications.co/view-article.php?doi=jmolgene.2019.1.13
KW  - Safe-food
KW  -ezymes
KW  -technique
KW  -cells produced proteins
KW  -agriculture
KW  -society
AB  - Human beings want enjoying highest attainable standard of basic health without discrimination of
race, religion and political belief, economic or social condition. The benefits of medical, psychological and
related knowledge have to be extended all people to the fullest attainment of health. Health is a state of
complete physical, mental and social well-being and not merely the absence of disease or infirmity. Three
factors affect human life greatly by: deficiency of food, health problems and environmental issues. Beside a
clean and safe environment, food and health are basic human requirements. Humans want safe-food at
reasonable price. Numerous human related health issues across the world cause huge number of deaths.
Therefore, the objective of this study is to review techniques and benefits of recombinant DNA technology.
Recombinant DNA technology is an important development in science that has made the human life much
easier. Two major categories of enzymes are important tools in the isolation of DNA and the preparation of
recombinant DNA technique are restriction endonucleases and DNA ligases. Recombinant DNA techniques
has seven steps (isolation of gene, preparation of target DNA insertion of DNA into vector insertion of plasmid
back into cell, plasmid multiplication, target cell production and cells produced proteins). Cloning vectors such
as YACS, bacteriophage, BACS, cosmids and plasmids are used for cloning of DNA. Recombinant DNA
technology has numerous applications in agriculture, health and diseases and environmental applications. It
is expected from, agricultural, medical and environmental sectors to work on this field, train professionals and
introduce its uses to the society.
ER  -