TY  - JOUR
T1  - Optimization the Production of the Sticky Ends of Human <I>CCRL1</I> Gene
AU - Karimian, Mohammad AU - Hashemi, Mohammad AU - Behjati, Mohadese AU - Esmaeili, Abolghasem 
JO  - Journal of Molecular Genetics
VL  - 3
IS  - 1
SP  - 5
EP  - 7
PY  - 2011
DA  - 2001/08/19
SN  - 2070-4267
DO  - jmolgene.2011.5.7
UR  - https://makhillpublications.co/view-article.php?doi=jmolgene.2011.5.7
KW  - taq polymerase
KW  -T-vector
KW  -white colony
KW  -blue colony
KW  -endonuclease
KW  -Iran
AB  - CCRL1 encoded by gene belongs to the family of G-protein coupled receptors. This protein is a kind of C-C chemokine receptors which has a basic role in evolutionary function of heart identified chemokines. The aim of this study is to improve the creation of sticky ends at <I>CCRL1</I> gene by T-vector. In this study, <I>CCRL1</I> gene was amplified by PCR. Restriction sites of BamHI and NotI enzymes were introdused into the 5&prime;end of the forward and reverse primers, respectively. Mentioned enzymes are in the endonuclease family group therefore can not create sticky ends at both sides of <I>CCRL1</I> gene to overcome this problem amplified fragment by Taq polymerase enzyme was inserted into T-vector. Then, recombinant vector was transformed in <I>E. coli</I>.
ER  -