TY  - JOUR
T1  - Serotype Identification and Detection of Related Genes of HPI Pathogenic Island of <I>Escherichia coli</I> Isolated from Pigs in Hebei Province of China
AU - Shi, Qiumei AU - Zhang, Yanying AU - Cheng, Shuqin AU - Gao, Guisheng AU - Fang, Hai AU - Gao, Guangping AU - Liu, Yuqin AU - Chen, Cuizhen AU - Wang, Qiang 
JO  - Veterinary Research
VL  - 6
IS  - 4
SP  - 99
EP  - 104
PY  - 2013
DA  - 2001/08/19
SN  - 1993-5412
DO  - vr.2013.99.104
UR  - https://makhillpublications.co/view-article.php?doi=vr.2013.99.104
KW  - E. coli from pigs
KW  -O serotype
KW  -high pathogenicity island
KW  -gene
KW  -O38
AB  - A total of 54 samples including duodenum, small intestine contents, lymphonodi mesenterici and diarrhea feces were collected from pigs died of diarrhea in Hebei Province of China in 2009~2011. These samples were examined for the presence of <I>E. coli</I> and serotype identification. High pathogenicity island was detected from <I>E. coli </I>isolates. The isolation and identification of O serotype of <I>E. coli</I> were conducted by common method, <I>fyuA</I> and <I>irp2</I> genes were detected using PCR. The 54 <I>E. coli</I> strains referred to ten serotypes, O38 was the dominant serotype whose proportion was 51.5% (17/33). The positive rate of <I>fyuA</I> gene was 24.4% (11/45), <I>irp2</I> was 42.2% (19/45), <I>irp2</I> and <I>fruA</I> was 13.3% (6/45).
ER  -