TY  - JOUR
T1  - Molecular Detection, Virus Isolation and Pathotyping of a Newcastle Diseaese 
  Virus Field Strain from Backyard Chickens in Qatar
AU - Mohran, K.A. AU - Haroun, M. AU - Hassan, M. 
JO  - Research Journal of Poultry Sciences
VL  - 4
IS  - 3
SP  - 28
EP  - 32
PY  - 2011
DA  - 2001/08/19
SN  - 1993-5285
DO  - rjpscience.2011.28.32
UR  - https://makhillpublications.co/view-article.php?doi=rjpscience.2011.28.32
KW  - nested RT-PCR
KW  -RT-PCR
KW  -chicken
KW  -pathotyping
KW  -newcastle disease virus
KW  -Newcastle diseases
KW  -virus isolation
AB  - A first trial of detection, isolation and pathotyping of a Newcastle Disease Virus (NDV) field strain was conducted in Qatar. NDV RNA was isolated, amplified and detected from oropharyngeal and cloacal swabs from clinically diseased backyard chickens and Amnioallantoic fluids (AAF) of infected chicken embryos using primary RT-PCR, nested RT-PCR and gel electrophoresing techniques. The 356 and 216 bp RNA fragments were retrieved in primary and nested RT-PCR, respectively. NDV was isolated, identified and pathotyped in commercial chicken embryos using Haemagglutination (HA), Haemagglutination Inhibition (HI) tests, Mean Death Time (MDT) and Intracerebral Pathogenicity Index (ICPI). The causative agent was found to be a highly virulent strain.
ER  -