TY  - JOUR
T1  - Secondary Structures and Phylogenetic Relationship in the IGF 1 Gene Sequence of Three Local Chicken Strains
AU - Isaac, L.J. AU - Okon, B. AU - Ibom, L.A. AU - Dauda, A. 
JO  - Research Journal of Animal Sciences
VL  - 19
IS  - 1
SP  - 1
EP  - 6
PY  - 2025
DA  - 2001/08/19
SN  - 1993-5269
DO  - makrjans.2025.1.1.6
UR  - https://makhillpublications.co/view-article.php?doi=makrjans.2025.1.1.6
KW  - Secondary structure
KW  - phylogenetic
KW  - IGF-1
KW  - gene sequence
KW  - local chicken
KW  - strains
AB  - Secondary structure prediction is vital for determining protein’s structure
and functions, whereas phylogenetic analysis provides an understanding
of how species evolve due to genetic alterations. Since the IGF-1 gene is
a major participant in the developmental and reproductive abilities of
poultry and other animals, its potential as a molecular marker was thus
assessed. Sixty local chicken strains-normal feathered, frizzle feathered
and naked neck-comprising twenty from each strain, from which fifteenfive
was sampled per strain for blood collection and DNA extraction)
were involved in the work. Jena Bioscience Gmbh preparation kit was
used in extracting DNA, while the Shine Gene Primers given by:
GTCGGGCTACTTGAGTTACTAC-Forward. TTGCGCAGGCTCTATCT GCTCReverse,
was used to identify genomic DNA for sequencing of the gene
(IGF-1). About 2% agarose gel was used to assess the DNA purity. Results
showed variation in the secondary structure of IGF-1 gene in these strains
with the naked neck having a higher percentage of alpha helix, beta turn
and random coil than the others and the mingling of the strains with
other Gallus species in the phylogenetic tree. This is an indication of its
ability to withstand mutation which makes it stable. Thus, making IGF-1
a marker of interest in the genomic selection of chicken for development
and improvement.
ER  -