TY  - JOUR
T1  - PCR Assay for Experimental Detection of <I>Streptococcus agalactiae</I> Based on <I>ScpB</I> Gene in Rainbow Trout (<I>Oncorhynchus mykiss</I>)
AU - Monfared, S. Rashidi AU - Nematollahi, M.A. AU - Mirvaghefi, A.R. AU - Farahmand, H. AU - Bakhsh, S.A. Pour 
JO  - Journal of Fisheries International
VL  - 6
IS  - 4
SP  - 75
EP  - 79
PY  - 2011
DA  - 2001/08/19
SN  - 1817-3381
DO  - jfish.2011.75.79
UR  - https://makhillpublications.co/view-article.php?doi=jfish.2011.75.79
KW  - Streptococcus agalactiae
KW  -Oncorhynchus mykiss
KW  -scpB gene
KW  -rainbow trout
KW  -rainbow trout
KW  -mortality
KW  -Iran
AB  - <I>Streptococcus agalactiae</I> (Group B, GBS) is an important human and animal pathogen that causes invasive infection in fish. Present research was conducted by PCR experimental detection of <I>S. agalactiae</I> using <I>scpB</I> gene. One group of 48 fishes were challenged intramuscular (i.m.) with 0.5 mL bacterial suspension containing of 3.5x10<SUP>7</SUP> CFU/0.5 mL with normal saline and compared with control group that were injected by 0.5 mL sterile saline. Kidney tissue was selected for testing. Results showed that in 26 days, mortality ranged was 9.37% in challenged group. <I>S. agalactiae </I>was successfully detected by PCR in 42 challenged samples (87.5%).
ER  -