TY  - JOUR
T1  - PHAs Accumulation in Bacterial Cells Isolated from Rice By-Products
AU - Chansatein, Onuma AU - On-Ong-Arj, Parichat 
JO  - Journal of Engineering and Applied Sciences
VL  - 14
IS  - 11
SP  - 3728
EP  - 3731
PY  - 2019
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2019.3728.3731
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2019.3728.3731
KW  - Polyhydroxyalkanoates (PHAs)
KW  -Accumulation
KW  -Rice by-products
KW  -Rice Barn (RB)
KW  -Thai Rice
Vermicelli (TRV)
KW  -Broken Rice
AB  - Polyhydroxyalkanoates (PHAs) are biopolymers synthesized by microorganisms. Bacteria can
accumulate PHAs in their cells and used as energy resource. In this study, PHAs-producing bacteria were
isolated from rice by-products particularly Glutinous Broken Rice (GBR), non-glutinous Broken Rice (BRR), Rice
Barn (RB) and Thai Rice Vermicelli (TRV). The samples were cultured on three cultural media including of
Tryptic Soy Agar (TSA), Plate Count Agar (PCA) and Starch Agar (SA). The 356 of purified isolates were
cultured on complex and minimal medium then screened for PHAs accumulation in their cells using Nile Red
dyeing method. Detection of the intracellular granules was using florescent and transmission electron
microscopy. Isolate BRR25-1 and RB36 were accumulated large areas of PHAs inclusion in their cells when
cultured on SA and TSA, respectively. The isolates were sequenced based on 16S rRNA gene and submitted
in GenBank with Accession No. MG745381 and MG745383. The potential isolates could be produced and
accumulated PHAs in their cells when fermented in cheap carbon substrates from rice by-products.
ER  -