TY  - JOUR
T1  - Comparison of Three Schemes of Quantum Dots-Based Immunochromatography for
Serodiagnosis of Brucellosis in Cattle
AU - Dzantiev, Boris B. AU - Mukantayev, Kanatbek N. AU - Ramankulov, Yerlan M. AU - Mukanov, Kassym K. AU - Eskendirova, Saule Z. AU - N. Berlina, Anna AU - Zherdev, Anatoly V. AU - V. Sotnikov, Dmitriy 
JO  - Journal of Engineering and Applied Sciences
VL  - 14
IS  - 11
SP  - 3711
EP  - 3718
PY  - 2019
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2019.3711.3718
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2019.3711.3718
KW  - lateral flow immunoassay
KW  -immunochromatography
KW  -quantum dots
KW  -Serodiagnosis
KW  -superoxidedismutase
KW  -Brucellosis
AB  - Three schemes of immunochromatographic serodiagnosis are considered and compared
experimentally to determine the specific antibodies that act against the recombinant antigen of Brucella abortus
(superoxide dismutase). The considered schemes differ in the order of immunoreagents interacting with the
membranes and accordingly in the composition of the detected complexes. As a label, fluorescent nanocrystals
were used quantum dots which allow more sensitive detection than traditional gold nano particles. The
manufactured test systems were used to test the samples of blood sera of cows with confirmed Brucellosis. The
obtained results demonstrated that the highest signal value is achieved in the scheme when the antigen is
immobilized on the quantum dot’s surface and on the working membrane of the test system and the detected
complexes are formed due to the presence of several valencies in antibodies.
ER  -