TY  - JOUR
T1  - Indirect Response of Early Mouse Embryo to Oxidative Stress Evoked in Apoptosis Model
AU - Suvorov, O.A. AU - Panait, A.I. AU - Bakhir, V.M. AU - Pogorelov, A.G. AU - Pogorelova, M.A. AU - Ivanisky, G.R. 
JO  - Journal of Engineering and Applied Sciences
VL  - 13
IS  - 12
SP  - 4300
EP  - 4304
PY  - 2018
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2018.4300.4304
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2018.4300.4304
KW  - Oocyte and early embryo
KW  -NMRI mouse
KW  -apoptosis
KW  -hydrogen peroxide
KW  -electrochemically reduced
KW  -quantitative laser microtomography
KW  -cellular volume
KW  -Oocyte and early embryo
KW  -NMRI mouse
KW  -apoptosis
KW  -hydrogen peroxide
KW  -electrochemically reduced
KW  -quantitative laser microtomography
KW  -cellular volume
AB  - Quantitative laser microtomography was employed to measure the single cell volume. NMRI mouse
zygotes and 2-cell embryos were shown to exhibit the shrinkage at apoptosis induced with ROS (40 min., H<sub>2</sub> O<sub>2</sub> , 
0.2 mM). Unlike early embryos, there was no influence of<i> in vitro</i> apoptotic circumstances on mature oocytes,
direct precursor of early embryogenesis. Dulbecco&#146;s solution prepared from electrochemically reduced
bi-distilled water allows the low redox potential in both normal and apoptotic conditions. The data obtained
permit us to suggest that ROS sub-milimolar concentrations likely act as the signaling impact. Cys-loo receptors
containing thiol group may be considered as the target of hydrogen peroxide molecules.
ER  -