TY  - JOUR
T1  - A Potential Strain RB36 of Polyhydroxyalkanoates-Producing Bacterium
Isolated from Rice Barn Located in Nakhon Ratchasima Province, Thailand
AU - Chansatein, Onuma 
JO  - Journal of Engineering and Applied Sciences
VL  - 13
IS  - 11
SP  - 4245
EP  - 4249
PY  - 2018
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2018.4245.4249
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2018.4245.4249
KW  - Potential strain
KW  -polyhydroxyalkanoates and rice barn
KW  -potential
KW  -bacterium
KW  -rice
KW  -electron
AB  - Polyhydroxyalkanoates (PHAs) are groups of biodegradable thermoplastic polymers synthesized by
various microorganisms. PHA producing bacteria accumulate PHA in their cells as carbon and energy storage
compounds. This research objective was to acquire a strain of PHA producing bacterium isolated from rice barn.
Rice barn samples were collected from rice mills at Nakhon Ratchasima Province, Thailand. Bacterial PHA
production was isolated on Tryptic Soy Agar (TSA), cultured on complex and minimal medium agar and then
screened on minimal medium agar supplemented with Nile Red. PHA accumulation in bacterial cells was
detected by Transmission Electron Microscopy (TEM) and fluorescent microscopy on samples stained with
Nile Blue A. The 40 of 214 isolates produce PHAs in their cell at rates from 5.1-76%. Bacterial strain RB36
produced PHAs in as much as 76% of cell areas. The strain was sequenced based on 16S ribosomal RNA gene
and then submitted in GenBank with Accession No. MG745383 and identified as 99.8-100% similarity to enter
ococcus faecium. This is the first report of a potential strain of PHAs-producing bacterium isolated from rice
barn from Thailand.
ER  -