TY  - JOUR
T1  - Comparative Effects Between Green Tea and Black Tea Polyphenols in Suppressing
Adverse Effects of TNF-α Induced Inflammation in Osteoblasts
AU - Mohd Ismail, Aletza AU - Zulkipli, Husna AU - Froemming, Gabriele Ruth Anisah 
JO  - Journal of Engineering and Applied Sciences
VL  - 13
IS  - 6
SP  - 1552
EP  - 1560
PY  - 2018
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2018.1552.1560
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2018.1552.1560
KW  - Black tea
KW  -polyphenols
KW  -chronic inflammation
KW  -osteoblasts
KW  -OPG
KW  -RANKL
AB  - The aim of the study was to compare the osteoprotective Effects of Green Tea (GTE) and Black Tea
(BTE) Extracts on Normal Human Osteoblast (NHOst) cells in non-inflammatory and inflammatory conditions.
NHOst cells were treated with GTE and BTE 5, 10, 50 and 100 μg/mL for 2, 5 and 10 days. The experiments were
performed in the absence and presence of Tumour Necrosis Factor-α (TNF-α) to emulate non-inflammatory and
inflammatory conditions, respectively. All concentrations of GTE and BTE exhibited = 80% cell proliferation at
all-time points. In the absence of TNF-α, 5 μg/mL of GTE and BTE significantly up-regulated Osteo Prote Gerin
(OPG) level compared to control and 100 μg/mL of the extracts reduced Receptor Activator of Nuclear factor
Kappa B Ligand (RANKL) level on day 5 and 10. In inflammation, 5 and 50 μg/mL BTE significantly elevated
OPG level while with GTE only 5 μg/mL gave a similar effect. Higher concentrations 50 and 100 μg/mL of both
extracts significantly suppressed RANKL expression. The 100 μg/mL GTE and all BTE concentrations tested
except 100 μg/mL significantly increased Alkaline Phosphatase (ALP) activity by day 5 in non-inflammatory
condition. About 5 μg/mL GTE increased the ALP activity in inflammatory condition. Likewise, BTE was also
found to reverse the TNF-α effect by elevating the ALP activity. GTE and BTE increased formation of
mineralized nodules in both conditions at each time points. BTE and GTE exert protective effects on osteoblast
activities including reverting the TNF-α-induced adverse effects and these effects are more pronounced in BTE
treatment.
ER  -