TY  - JOUR
T1  - Lipopolysaccharide-Induced Alterations in mRNA Expression of Genes Related to Fatty Acid Metabolism
AU - Jiaqi, Wang AU - Liansheng, Zhao AU - Nan, Zheng AU - Yangdong, Zhang AU - Xuemei, Nan AU - Han, Hu AU - Ruilian, Cui AU - Lingyun, Zhou 
JO  - Agricultural Journal
VL  - 9
IS  - 2
SP  - 99
EP  - 102
PY  - 2014
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2014.99.102
UR  - https://makhillpublications.co/view-article.php?doi=aj.2014.99.102
KW  - LPS
KW  -fatty acids metabolic-related gene
KW  -mammary epithelial cells
KW  -milk fat
KW  -China
AB  - The study was conducted to evaluate the effect of Lipopolysaccharide 
  (LPS) on milk fatty acid metabolism. The expression of genes for fatty acid 
  metabolism in dairy cows was studied by incubating mammary epithelial cells 
  with LPS at 0, 0.1 or 10 ng mL<SUP>-1</SUP> for 24 h using RT-qRCR <I>in vitro</I>. 
  LPS increased transporter CD36 mRNA expression (p&lt;0.01). FABP3 mRNA expression 
  (p&gt;0.05) was not affected by LPS but the FABP4 mRNA expression was affected 
  (p&lt;0.05), decreasing 0.79 fold when LPS was provided at 10 ng mL<SUP>-1</SUP>. 
  Treatment with 0.1 ng mL<SUP>-1</SUP> LPS tend to increased fatty acid synthesis 
  enzyme FASN, ACACA, ACSS2 and fatty acid metabolism gene PPARG, PPARGC1A mRNA 
  expression (0.10&gt;p&gt;0.05) compared with the control. LPS alters fatty acid 
  metabolism, functioning as an important factor affecting milk fat synthesis.
ER  -