TY  - JOUR
T1  - Establishment and Characterization of a Chicken Oviduct Epithelial Cell Line from Hy-Line Variety Brown
AU - Zhang, Qiuting AU - Wang, Chunsheng AU - Wu, Zhihao AU - Piao, Shanhua AU - Liang, Yang AU - An, Tiezhu 
JO  - Agricultural Journal
VL  - 9
IS  - 2
SP  - 86
EP  - 92
PY  - 2014
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2014.86.92
UR  - https://makhillpublications.co/view-article.php?doi=aj.2014.86.92
KW  - Oviduct epithelial cells
KW  -obturated interior digesting
KW  -chicken
KW  -cell line
KW  -egg albumin
AB  - Chicken oviduct epithelial cells synthesize and secret most 
  egg albumin can be a cell bioreactor or take part in preparation of intravital 
  bioreactor, also are a good model for studying chicken <I>oviductal</I> gene 
  expression regulation. Researchers established a chicken oviduct epithelial 
  cell line named XYF-142, meanwhile, developed a new method, obturated interior 
  digesting for efficiently procuring uncontaminated primary cells. The population 
  doubling time of XYF-142 was 106.67 h. About 92.5% of 5th XYF-142 cells were 
  diploid in chromosome analysis. The results of real-time PCR indicated that 
  the expression level of oviductal epithelia-specific gene-SERPINB3 was higher 
  in cells than in oviduct tissue and higher in 5th and 15th epithelia than in 
  primary epithelia. In immunofluorescence cytochemistry detection for XYF-142, 
  all cells expressed the marker protein-cell keratin 18. XYF-142 cells were competent 
  to be transfected with vector DNA and transfection efficiency can reach 60%. 
  Therefore, researchers concluded that researchers have successfully established 
  a chicken oviduct epithelialcell line, provided a model for studying gene expression 
  regulation and tissue-specific detection of chicken oviduct.
ER  -