TY  - JOUR
T1  - Genetic Characterization of Canine Parvovirus Isolates From Beijing of China 
  Between 2009 and 2011
AU - Wang, Jing AU - Shi, Li-Jun AU - Wang, Peng AU - Zhao, Zhan-Zhong AU - Gong, Miao-Miao AU - Li, Gang AU - Yuan, Wei-Feng AU - Zhu, Hong-Fei 
JO  - Agricultural Journal
VL  - 8
IS  - 2
SP  - 60
EP  - 65
PY  - 2013
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2013.60.65
UR  - https://makhillpublications.co/view-article.php?doi=aj.2013.60.65
KW  - Canine parvovirus
KW  -VP2 gene
KW  -phylogenetic analysis
KW  -amino acid
KW  -Beijing
KW  -China
AB  - In order to investigate the new strains circulating in Beijing, 24 positive samples of CPVs were isolated from Beijing of China between 2009 and 2011 and the sequences of the main capsid protein VP2 were obtained. These sequences were analyzed in comparison with the sequences of 29 other strains of CPV from GenBank. Two types of CPV, including CPV-2 and CPV-2a were detected and CPV-2a (with Ala297 mutation) was predominant in Beijing and 4 strains CPV-2a types were found to carry the amino acid substitution Ala440. The unique Ile324 mutation in the VP2 of Beijing CPV isolate was detected as compared with a Tyr324 in the VP2 of the reference CPV strains. A phylogenetic tree was constructed from the <I>VP2</I> genes. These results showed that most of the isolated Beijing strains classified in a cluster Chinese field isolates which were distinct from American and Italian isolates.
ER  -