TY  - JOUR
T1  - Isolation, Sequence Identification and Tissue Expression Profile of a Novel Goat Gene <I>RHOA</I>
AU - Jing-Hu, Zhang AU - Wei-Hua, Gong AU - Hong-Wen, Yang 
JO  - Agricultural Journal
VL  - 7
IS  - 3
SP  - 194
EP  - 197
PY  - 2012
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2012.194.197
UR  - https://makhillpublications.co/view-article.php?doi=aj.2012.194.197
KW  - Goat
KW  -RHOA
KW  -RACE
KW  -tissue expression profile
KW  -sequence identification
KW  -China
AB  - The full-length cDNA sequence of one goat gene <I>RHOA</I> was amplified using the rapid amplification of cDNA ends (RACE) method based on one goat EST sequence which was highly homologous to the coding sequence of human <I>RHOA</I> gene. Sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 193 amino acids that shares high homology with the ras homolog gene family, member A (<I>RHOA</I>) of nine species human (100%), cattle (100%), sheep (100%), rat (99%), mouse (99%), chicken (99%), dog (99%), turkey (99%) and green anole (99%) so that it can be defined as goat <I>RHOA</I> gene. This novel goat gene was then deposited into NCBI database. Phylogenetic analysis revealed that the goat <I>RHOA</I> gene has a closer genetic relationship with the <I>RHOA</I> genes of human, cattle and sheep. Tissue expression analysis indicated that the goat <I>RHOA</I> gene is generally and differentially expressed in detected tissues including spleen, muscle, skin, kidney, lung, liver, fat and heart. This experiment established the primary foundation for further research on the goat <I>RHOA</I> gene.
ER  -