TY  - JOUR
T1  - Effects of Crude Polysaccharides from <I>Catathelasma ventricosum</I> On the Proliferation and Differentiation of 3T3-L1 Cells
AU - Li, Xuewei AU - Wang, Xun AU - Liu, Haifeng AU - Zhao, Ling AU - Dong, Han AU - Wang, Yuhao AU - Sun, Di AU - Liu, Yuyin AU - Yang, Ruqi 
JO  - Agricultural Journal
VL  - 7
IS  - 3
SP  - 187
EP  - 190
PY  - 2012
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2012.187.190
UR  - https://makhillpublications.co/view-article.php?doi=aj.2012.187.190
KW  - Catathelasma ventricosum
KW  -polysaccharides
KW  -3T3-L1
KW  -proliferation
KW  -differentation
KW  -China
AB  - To study the effects of Crude Polysaccharides from <I>Catathelasma ventricosum</I> (CVCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture media contained different concentrations of CVCP (0, 150, 300 and 600 &#956;g mL<SUP>-1</SUP>) for 24 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Nile Red staining on days 8 after treatment with culture media supplemented with different concentration CVCP (0, 150, 300 and 600 &#956;g mL<SUP>-1</SUP>). As the results, the different concentration CVCP (150, 300 and 600 &#956;g mL<SUP>-1</SUP>) promoted proliferation of 3T3-L1 preadipocytes when compared with the control (p&lt;0.05). And it stimulated cell differentiation into adipocyte at low concentration (150 &#956;g mL<SUP>-1</SUP>) while it inhibited cell differentiation into adipocyte at higher concentration (300 and 600 &#956;g mL<SUP>-1</SUP>). But the difference was not significant (p&gt;0.05). Besides, lipid content in 3T3-L1 cells treated with 150 &#956;g mL<SUP>-1</SUP> was significantly higher than treated with 600 &#956;g mL<SUP>-1</SUP> (p&lt;0.05). It can be concluded from the results that the CVCP promoted proliferation of the 3T3-L1 cells 24 h after treatment. Meanwhile, it was involved to some extent in regulation of adipogenesis and lipogenesis.
ER  -