TY  - JOUR
T1  - Evaluation of Duplex PCR and PCR-RFLP for Diagnosis of Sheep Pox and Goat Pox
AU - , Sadanand Fulzele AU - , Raj Kumar Singh AU - , Madhusudan Hosamani AU - , Bimalendu Mondal AU - , Mahendra Pal Yadav 
JO  - International Journal of Tropical Medicine
VL  - 1
IS  - 2
SP  - 66
EP  - 70
PY  - 2006
DA  - 2001/08/19
SN  - 1816-3319
DO  - ijtmed.2006.66.70
UR  - https://makhillpublications.co/view-article.php?doi=ijtmed.2006.66.70
KW  - Goat pox
KW  -sheep pox
KW  -diagnosis
KW  -PCR
AB  - In the present study, we evaluated the diagnostic efficacy of attachment gene based PCR (A-PCR)
and A-PCR-RFLP employing a number of <I>capripoxvirus </I>field samples received from different parts of the
country during 2000-2005. The tests were compared with duplex PCR and A-PCR-RFLP methods. We also
cloned and sequenced A-PCR products from three representative Goatpoxvirus (GPV) isolates and three
representatives Sheeppox Virus (SPV) isolates. F-PCR products of two isolates of GPV were also cloned and
sequenced. Sequence variation in the attachment and fusion gene fragments of SPV and GPV was also
determined to confirm the differential amplification in the suspected clinical samples. Phylognetic analysis
based on partial fusion gene sequence of capripoxviruses showed that Indian isolates are closely related to
other GPV strains published earlier.
ER  -