TY  - JOUR
T1  - Comparison of Coventional Isolation, Phage-Based Assay and PCR for Detection of <I>Mycobacterium tuberculosis </I>Complex
AU - , Haitham A. Albir AU - , Suliman M. ElSanousi AU - , Tarig G. Eldawi AU - , Mohamed E. Ahmed AU - , Imadeldin E. Aradaib 
JO  - International Journal of Tropical Medicine
VL  - 1
IS  - 2
SP  - 48
EP  - 52
PY  - 2006
DA  - 2001/08/19
SN  - 1816-3319
DO  - ijtmed.2006.48.52
UR  - https://makhillpublications.co/view-article.php?doi=ijtmed.2006.48.52
KW  - Conventional isolation
KW  -mycobacterium tuberculosis
KW  -PCR
AB  - In the present study, conventional bacterial isolation, bacteriophage-based assay (FAST Plaque TB)
and Polymerase Chain Reaction (PCR) were evaluated for detection of <I>Mycobacterium tuberculosis </I>complex.
A total of 47 mycobacterial isolates consisting of 38 isolates of <I>Mycobacterium tuberculosis </I>complex and 9
isolates of mycobacteria other than <I>M. tuberculosis </I>complex were used in this study. In addition, nine reference
strains of Mycobacterium consisting of 7 <I>Mycobacterium tuberculosis, </I>one strain <I>of Mycobacterium
flavescen</I>s, one srtrain of <I>mycobacterium duvalii </I>were also used in this study. Conventional isolation is
laborious, cumbersome and time consuming where it takes as long as 2 months for definitive diagnosis. The
phage assay is sensitive and it takes only two working days. PCR is a rapid assay and definitive diagnosis of
tuberculosis infection could be made possible within the same working day. The described
bacteriophage-based assay could be used as rapid, sensitiveand specific method to support the currently
available conventional methods used for detection of <I>Mycobacterium tuberculosis </I>in developing countries.
ER  -