TY  - JOUR
T1  - The Pattern of Potassium and Protein Leakage from Microbial Cells by <I>Vernonia tenoreana </I>Leaf Extract
AU - , A.O. Ogundare 
JO  - International Journal of Tropical Medicine
VL  - 1
IS  - 3
SP  - 100
EP  - 102
PY  - 2006
DA  - 2001/08/19
SN  - 1816-3319
DO  - ijtmed.2006.100.102
UR  - https://makhillpublications.co/view-article.php?doi=ijtmed.2006.100.102
KW  - Extracts
KW  -leakage
KW  -potassium ion
KW  -protein
KW  -organisms
AB  - The mechanism of action of the leaf extracts of <I>V. tenoreana</I> on some pathogens was investigated. The   pathogens  included <I> Staphylococcus  aureus </I>(NCIB 67), <I> Bacillus subtilis </I> (NCIB3610), <I>Pseudomonas aeruginosa </I>(NCIB 950) and <I>Escherichia coli</I> (NCIB 86). Crude extract of  the leaf was obtained using methanol and distilled water (in ratio 2:3 v/v).   The extract leaked potassium and protein from the cells of <I>S. aureus, B. subtilis, E .coli</I> and <I>Pseudomonas aeruginosa</I>. Protein was leaked increasingly from the bacterial cells during the first 60 mins of interaction, after which a continuous decrease in the  protein leakage began. However, the leakage of potassium ions from the cells of the pathogen followed an increasing trend throughout. The leaf extract<I> </I>leaked 0.30 M of potassium from <I>S. aureus</I> at ten minutes interaction, while as much as 103.87 M was leaked at 60 min interaction from the same organism.
ER  -