TY  - JOUR
T1  - <I>In vitro</I> Response of Cancer Cells to the Growth-Inhibitory Effects of Dichloromethane Extract of <I>Goniothalamus umbrosus</I>
AU - , Ahmad Bustamam Abdul AU - , Siddig Ibrahim Abdel-Wahab AU - , Hew Kim Fong AU - , Syam Morali Mohan AU - , Adel Sharaf Al-Zubairi AU - , Manal Mohamed Elhassan 
JO  - Research Journal of Pharmacology
VL  - 3
IS  - 1
SP  - 1
EP  - 6
PY  - 2009
DA  - 2001/08/19
SN  - 1815-9362
DO  - rjpharm.2009.1.6
UR  - https://makhillpublications.co/view-article.php?doi=rjpharm.2009.1.6
KW  - Goniothalamus umbrosus
KW  -breast cancer
KW  -apoptosis
KW  -MCF-7
KW  -HT-29
AB  - This present study aims to investigate whether <I>Goniothalamus umbrosus</I> extract has anticancer effects on several human cancer cells namely, the MCF-7 breast cancer cell line, HT-29 colon cancer cell line and CEM-ss leukemia cell line. Morphological changes and probable mode of cancer cell death induced by these <I>G. umbrosus</I> extract was examined. The dichloromethane plant extract of <I>G. umbrosus</I> was investigated for its cytotoxic activities on the human cancer cells using a 3 days MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The MTT assay results revealed that the extract has anticancer effects on human breast cancer cells (MCF-7). Half maximal inhibitory concentration (IC<SUB>50</SUB>) of the hexane extract was found to be 19.5&plusmn;0.338 mg mL<SUP>-1</SUP> and this concentration was further used in investigations towards MCF-7 human breast cancer cells. Both inverted and fluorescence microscopic studies demonstrated that treated MCF-7 breast cancer cells using IC<SUB>50</SUB> of the extract displayed a number of typical morphological changes. Appearance of membrane blebs, DNA condensation and fragmentation are significant signs of apoptosis. The above findings suggested, that the hexane extract of <I>Goniothalamus umbrosus</I> has potential therapeutic effect towards human breast cancer cells that requires further investigations in future.
ER  -