TY  - JOUR
T1  - Development of Sensitive High-Specific Rapid Tests Based on Dna Markers for the Diagnosis
of Pathogenic Fungi of genera Puccinia and Pyrenophora, Causing Diseases of Cereals
AU - Beishova, I. AU - Chuzhebaeva, G. AU - Ulyanov, V. AU - Zharlygassov, Zh. AU - Sultangazina, G. AU - Stakheev, A. AU - Zavriev, S. 
JO  - Research Journal of Medical Sciences
VL  - 14
IS  - 5
SP  - 84
EP  - 88
PY  - 2020
DA  - 2001/08/19
SN  - 1815-9346
DO  - rjmsci.2020.84.88
UR  - https://makhillpublications.co/view-article.php?doi=rjmsci.2020.84.88
KW  - Quantitative PCR
KW  -primers
KW  -probes
KW  -Pyrenophora tritici-repentis
KW  -Puccinia graminis
KW  -Puccinia recondita and ITS
AB  - Traditional diagnostic methods, such as
enzyme-linked immunosorbent assays and microscopic
diagnostics, require quite a lot of time do not have the
necessary high sensitivity and do not always provide
unambiguous identification of species. Therefore, the
creation of test systems that allow the detection of
pathogens quickly and accurately remains an urgent task.
The Polymerase Chain Reaction (PCR) method is more
sensitive and specific in comparison with microbiological
methods and its real-time modification (quantitative PCR)
using a hybridization probe with a fluorescent label makes
it possible to quickly analyze the results without
performing electrophoresis.
ER  -