TY  - JOUR
T1  - Molecular Characterization and Inhibition by Natural Agents of Multidrug Resistant Candida Strains Causing Vaginal Candidiasis
AU - Enan, Gamal AU - Atef, Dina AU - Amer, Mahmoud AU - Mahdy, Ahmed AU - I. EL-Sayed, Talaat 
JO  - Research Journal of Medical Sciences
VL  - 9
IS  - 1
SP  - 1
EP  - 7
PY  - 2015
DA  - 2001/08/19
SN  - 1815-9346
DO  - rjmsci.2015.1.7
UR  - https://makhillpublications.co/view-article.php?doi=rjmsci.2015.1.7
KW  - Genetic linkage
KW  -Candidiasis
KW  -Aqueous extract of alum and clove
KW  -Antifungal resistance
KW  -Candida spp.
KW  -Identification
AB  - The prevalence percentage of vaginal candidiasis appeared to be 49% within Egyptian pregnant women suffering from vaginal pain and vaginal discharge. The 61.2% of the 49 patients were multigravidae and almost in the age range 20-30 years. Based on phenotypic and biochemical characteristics, the infective 49 isolates causing vaginal candidiasis could be classified and identified to C. <I>albicans</I> group (32 isolates), C. <I>tropicalis group</I> (12 isolates) and C. <I>dubliniensis</I> group (5 isolates). Three strains of them viz. C. <I>albicans</I> ZUH<SUB>1</SUB> , C. <I>trobicalis</I> ZUH<SUB>13</SUB> and C. <I>dubliniensis</I> ZUH<SUB>9</SUB> were resistant to 6 antifungal agents used and the antifungal resistance ability in each of them was genetically encoded and linked to one plasmid in each of them, almost in the range 600-800 bp. Of many natural agents used as antifungals, aqueous extracts of alum and clove inhibited vigorously the antifungal resistant strains: C. <I>albicans</I> ZUH<SUB>1</SUB> , C. <I>trobicalis</I> ZUH<SUB>13</SUB> and C. <I>dubliniensis</I> ZUH<SUB>9</SUB> ; no colony forming units were detected onto Sabouraud agar after 5 days of incubation from samples 9 treated with either alum or clove extracts.
ER  -