TY - JOUR T1 - The Effect of Salbutamol on the Parotid Salivary Gland of Albino Rats (Immunohistochemical Study) AU - Metwally, E.H. AU - El-Hawary, Y.M. AU - Grawish, M.A. AU - Mohammed, M.A. JO - Research Journal of Medical Sciences VL - 7 IS - 1 SP - 13 EP - 19 PY - 2013 DA - 2001/08/19 SN - 1815-9346 DO - rjmsci.2013.13.19 UR - https://makhillpublications.co/view-article.php?doi=rjmsci.2013.13.19 KW - p53 KW -Salbutamol KW -Parotid gland KW -ki-67 KW -Egypt AB - Salbutamol is a short-acting selective β2-adrenergic receptor agonist used for the relief of bronchospasm in conditions such as asthma and chronic obstructive pulmonary disease. The β-adrenoceptors are also present in the secretory system and have a strong impact on salivary composition. This study was done to evaluate the effect of the selective β2-adrenergic agonist salbutamol on the parotid salivary glands regarding cellular proliferation and apoptosis during long term treatment and after stoppage by immunohistochemical methods. One hundred male Albino rats were divided into control and study groups. The study group received daily injections of 4 mg kg-1 salbutamol (Ventolin, GSK) while the control group received equal amounts of saline. Rats were sacrificed at periods of 2, 7, 14 and 25 days of treatment and then 1 week after cessation of treatment. At each period the parotid glands were dissected and processed for H&E stain and immunohistochemical staining with ki-67 and p53 antibodies. After 2 days of treatment marked hypertrophy of the acini was noticed as well as marked increase in the number of proliferating cells as denoted by ki-67 antibody. Ducts and some endothelial cells showed increased proliferative activity. The proliferative activity decreased dramatically after that but did not go back to the normal level of the control. Apoptotic activity denoted by the p53 apoptotic marker showed an increase at 7 days post treatment. ER -