TY  - JOUR
T1  - <I>In vitro</I> Cytotoxic Pentacyclic Triterpenoids of <I>Newbouldia laevis</I>
AU - Onocha, P.A. AU - Okorie, D.A. AU - Krebs, H.C. AU - Meier, B. 
JO  - Research Journal of Medical Sciences
VL  - 5
IS  - 6
SP  - 330
EP  - 335
PY  - 2011
DA  - 2001/08/19
SN  - 1815-9346
DO  - rjmsci.2011.330.335
UR  - https://makhillpublications.co/view-article.php?doi=rjmsci.2011.330.335
KW  - Bignoniaceae
KW  -fibroblasts
KW  -cytotoxicity
KW  -bioactivity
KW  -ursenoic acid
KW  -oleanenoic acid
AB  - Bioactivity directed fractionation afforded four cytotoxic constituents from the bioactive methanolic leaf extract (LD<SUB>50</SUB> 30 &#956;g mLG<SUP>1</SUP>) of<I> Newbouldia laevis</I> (Bignoniaceae) collected from Ibadan, Nigeria. Four pentacyclic triterpenoids namely 2&#945;, 3&#946;, 19&#945;-trihydroxy-12-ursen-28-oic acid, 3&#946;, 19&#945;-dihydroxy-12-ursen-28-oic acid, 3&#946;-hydroxy-12-ursen-28-oic acid, 3&#946;-hydroxy 12-oleanen-28-oic acid were found to be the cytotoxic principles. These compounds demonstrated significant cytotoxicity <I>in vitro</I> against ST-57 brain tumor transformed fibroblasts with LD<SUB>50</SUB> ranging from 0.4 &#956;g mLG<SUP>1</SUP> (compound 1) to 4 &#956;g mLG<SUP>1</SUP> (compound 4). These ursene and oleanene type triterpenoids are encountered for the first time in this plant. The structures of these compounds were established by spectral data.
ER  -