TY  - JOUR
T1  - Fragile X Allelemorphism among the Mentally Retarded and Affected Families
AU - , A. Latunji Charles 
JO  - Research Journal of Medical Sciences
VL  - 2
IS  - 5
SP  - 224
EP  - 230
PY  - 2008
DA  - 2001/08/19
SN  - 1815-9346
DO  - rjmsci.2008.224.230
UR  - https://makhillpublications.co/view-article.php?doi=rjmsci.2008.224.230
KW  - Fragile X syndrome
KW  -mental retardation
KW  -karyotype
KW  -femal carriers
KW  -molecular analysis
AB  - The fragile X mental retardation allele was investigated in Ibadan, south west Nigeria. Blood specimens from a population of 659 Mentally Retarded individuals (MRs) were screened for the fragile X mutation using cytogenetic and molecular methods. It was observed that 235 (35.7%) individuals had chromosomal aetiologies to their mental impairment. The Down syndrome was highest occurring in 146 (21.2%) individuals, followed by the fragile X karyotype with 45 (6.83%) individuals expressing the 46, Xq 27.3 fragility. Of the fragile X individuals, there were 13 (2.0%) fragile X females, 2 of which were 45, XO/46, XX and 45, XO/46, XX/47, XXX mosaics, respectively. Molecular methods confirmed the cytogenetic findings, where affected individuals expressed the of trinucleotide repeat amplification in the order of >200 CGG repeats in the fragile X  allele region. Triplet repeat bands ranged between 200 and 2000 CGGs. Eight pedigrees comprising 70 normal relatives of 8 fragile X mentally retarded propositi were permissible to investigations for the determination of interfamilial transmission of the fragile X alleles. Blood samples were equally obtained from them and analysed, using cytogenetic and molecular methods likewise. Two normal sisters of a male propositus exhibited the 45, XO/47, XXX and 46, Xr(X)/45,  XO  mosaicisms, respectively. Molecular  analysis  revealed 26 (33.3%) female permutation carriers and 11 (14.1%) normal transmitting males. Eighteen (23.1%) males had normal alleles thus non-transmitting males and 15(19.2%) females were normal. The proportions observed in this survey, has implications for the general population and should prove significantly useful for clinicians and genetic counsellors.
ER  -