TY  - JOUR
T1  - Microbiological Investigation of Stool in Patients with Acute Diarrhea
AU - , Turan Gunduz AU - , Sedat Cumen AU - , Ali Ari AU - , Mete Demirel AU - , Serdag Etiz AU - , Ziya Tay 
JO  - Research Journal of Medical Sciences
VL  - 1
IS  - 2
SP  - 84
EP  - 87
PY  - 2007
DA  - 2001/08/19
SN  - 1815-9346
DO  - rjmsci.2007.84.87
UR  - https://makhillpublications.co/view-article.php?doi=rjmsci.2007.84.87
KW  - Diarrhea
KW  -Escherichia coli 0157
KW  -Salmonella sp.
KW  -Shigella sp.
KW  - G. intestinalis
AB  - Acute gastroenteritis is an important health public issue especially in developing countries. The aim of this study was to determine the most common microbial agents responsible for acute diarrheas in our city. The study was performed prospectively between December 2005 and January 2006 in Manisa, Turkey. Stool samples were collected from patients with acute diarrhea who attended to different hospitals of the city (University Hospital, Government Hospitals, Pediatrics Hospital and Health Public Laboratory). The stool samples of 300 patients (50 children, 250 adults) were investigated. Standard cultivation methods were performed to determine <I>Salmonella </I>ve <I>Shigella</I> sp<I>. E. coli </I>0157:H7 was tested by Immuno Card STAT! <I>E. coli </I>0157 Plus. The strains that were identified as sorbitol negative <I>E. coli </I>were investigated by <I>E. coli</I> 0157: H7 antiserum.   The  isolated  microorganisms  were  as follows:  <I>Salmonella </I>sp. 7 (2.3%), <I>Shigella</I> sp. 5 (1.6 %), <I>G. intestinalis</I> 12 (4%), <I>Enterobius vermicularis</I> 5 (1.6%), <I>Rotavirus</I> 2 (0.6%) and sorbitol negative <I>E. coli</I> 18 (6 %).  Only one Sorbitol negative <I>E. coli</I> colony showed agglutination with <I>E. coli </I>0157:H7<I> </I>antiserum. The most common pathogens were identified as <I>G. intestinalis</I> in children and <I>E. coli</I> in adults. Sorbitol negative <I>E. coli</I> were isolated in 18 of the stool samples, and only one of them showed agglutination with <I>E. coli </I>0157:H7. Although rare, this strain may lead serious complications and it should be kept in mind in patients with acute diarrhea.
ER  -