TY  - JOUR
T1  - Phenotypic and Genotypic Characterization of Antimicrobial Resistance among Diarrheagenic <I>Escherichia coli</I>
AU - , N. ALHaj AU - , N.S. Mariana AU - , A.R. Raha AU - , Z. Ishak 
JO  - Research Journal of Medical Sciences
VL  - 1
IS  - 5
SP  - 245
EP  - 250
PY  - 2007
DA  - 2001/08/19
SN  - 1815-9346
DO  - rjmsci.2007.245.250
UR  - https://makhillpublications.co/view-article.php?doi=rjmsci.2007.245.250
KW  - Antimicrobial Resistance (AMR)
KW  -Polymerase Chain Reactions (PCR)
KW  -Escherichia coli (E. coli)
KW  -public health
AB  - Diarrhea caused by multidrug-resistant bacteria is an important public health problem among children in developing countries. <I>Escherichia coli </I>is an important cause of disease in animals and humans worldwide. Twenty five <I>E. coli </I>isolates with rate 61.2% among human and environments were tested for susceptibility to 10 antimicrobial agents by disk diffusion method. Resistant isolates were screened by molecular methods for resistance genes, <I>TetA, TetB, </I>strepA, <I>MarI</I> and <I>MarII</I>. Molecular result showed that all isolates harbored resistance gene for the <I>TetA, TetB, </I>strepA, MarI,  and  <I>MarII</I> even though the genotypic test showed sensitive to the drugs. <I>E. coli</I> isolates exhibit a wide repertoire of genetic elements to sustain antimicrobial pressure. The results of this study using pheno-genotypic techniques highlight the distribution of <I>E. coli </I>among human, animal, aquatic ecosystems and the potential public health threat of <I>E. coli </I>originating from municipal wastewater sources.
ER  -